Fig. 2

Rapamycin and rapamycin plus metformin worsened primary OA. Representative images of histology from the medial tibia are shown for each group (A; scale bars are 0.5 mm and 0.25 mm in 5× and 10× images, respectively). Histological images were graded for total OARSI score (B; n = 7 per group). The individual scores for articular cartilage structure (C), proteoglycan loss (D), and cellularity (E) are also shown. Data analyzed using a one-way ANOVA to detect differences between groups for all variables besides ACS and cellularity, for which differences were determined using the non-parametric Kruskal-Wallis test with Dunn’s multiple comparison test. *P < 0.05 vs Con