Fig. 1

In vivo imaging of K/BxN spontaneous and serum transfer arthritis using LS301. Arthritic or control (non-diseased) mice were injected intravenously with 6 nmol LS301. Whole body near-infrared fluorescence images were taken on the Pearl animal imaging system with λ = 785 nm excitation and λ = 820 nm emission filters. Images shown are representative of at least two independent experiments and were taken at 18 h post-injection with mice in dorsal orientation. A Early-stage spontaneous K/BxN arthritis (3–4 weeks old F1 mice). B Intermediate stage spontaneous K/BxN arthritis (5–7 weeks old F1 mice). C Late-stage spontaneous K/BxN arthritis (9–10 weeks old F1 mice). D C57BL/6J mice with serum transfer arthritis (day 4 post disease induction). E Normal (control) C57BL/6J mice injected with LS301. F Intermediate stage spontaneous K/BxN arthritis (5–7 weeks old) F1 mice injected with cypate dye (control) in lieu of LS301. Numbers denote individual clinical paw scores at the time of imaging