Fig. 1
From: Immune cell profiles in synovial fluid after anterior cruciate ligament and meniscus injuries
![Fig. 1](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13075-021-02661-1/MediaObjects/13075_2021_2661_Fig1_HTML.png)
Representative flow cytometry gating strategy for the broad spectrum panel. A To identify immune subsets, non-relevant events including debris, doublets, red blood cells, and dead cells were removed through gating using the Flowjo software. Gating on CD45 cells, B immune cells were then segregated into (1) B cells, (2) T cells, and (3) innate immune cells (non B and T cells). C Gating on the innate population from B3, cells were divided into neutrophils and monocyte (MO) subsets: classical, intermediate, and activated, which were gated off of total monocytes