Fig. 1

GSDMD deficiency did not reduce joint swelling and osteolysis induced by STIA. Six-week-old mice were injected intraperitoneally with PBS (Gsdmd^+/+: 4 females and 3 males; Gsdmd^−/−: 4 females and 2 males) or K/BxN mouse serum (Gsdmd^+/+: 3 females and 2 males; Gsdmd^−/−: 4 females and 3 males) on days 0 and 2. Ankle (A) and paw (B) thicknesses were measured daily for 12 days with a digital caliper. No differences in joint swelling were noted between male and female mice. C The hindlimbs were collected on day 12 and scanned. White arrows indicate the areas of extensive osteolysis. D, E qPCR analysis of Gsdmd mRNA expression in Gsdmd^+/+ paw tissues. Data are mean ± SD. Student’s t test; **p < 0.01; ***p < 0.001