Fig. 3

GSDMD was expressed in mouse joint tissues and synovium of human OA patients. A–F Representative images of specimens of Gsdmd^+/+ mice stained with GSDMD antibody. B, C, E, F Magnified views of the boxed areas in A and D. G–I Representative images of specimens of Gsdmd^−/− mice stained with GSDMD antibody. Brown and dark red show specific staining. Scale bar = 250 μm (A–I). AC, articular cartilage; BM, bone marrow; M, meniscus. J Immunoblotting analysis of GSDMD expression and cleavage in the synovium from 1 normal and 8 OA patients. GAPDH was used as a loading control