Fig. 5

GSDMD deficiency attenuated subchondral bone sclerosis. Twelve-week-old Gsdmd^+/+ and Gsdmd^−/− male mice were subjected to sham or MLI surgery. A Representative three-dimensional μCT figures of sham and MLI knee joints. “PET” color scheme from the Dragonfly software was used to highlight the subchondral bone changes. B Subchondral BV/TV changes. MLI BV/TV/Sham BV/TV percentage for each mouse was used to quantify the extent of subchondral bone sclerosis. Reference data are shown in Fig. S3B. C Subchondral bone plate thickness changes. MLI thickness/sham thickness percentage for each mouse was used to quantify the extent of subchondral bone sclerosis. Data are mean ± SD. N = 7–8/group. Unpaired t test; *p < 0.05; ***p < 0.001. Scale bar = 5 mm