Table 2 RT-qPCR primers
From: WNT16 elevation induced cell senescence of osteoblasts in ankylosing spondylitis
Gene | 5′-Forward-3′ | 3′-Reverse-5′ |
|---|---|---|
GAPDH | CAAGATCATCAGCAATGCC | CTGTGGTCATGAGTCCTTCC |
WNT16 | CTACGGAGCCCAAGGAAACT | CTCTCGTGTCTGAACTGGCT |
TP53 | ACCTATGGAAACTACTTCCTGAAA | CTGGCATTCTGGGAGCTTCA |
CDKN1A (p21) | GCAGAGGAAGACCATGTGGA | CGGCGTTTGGAGTGGTAGAA |
CASP3 | TCATTATTCAGGCCTGCCGT | GTCGGCCTCCACTGGTATTT |
IL1A | TGGTAGTAGCAACCAACGGG | GGTGCTGACCTAGGCTTGAT |
IL1B | CAGAAGTACCTGAGCTCGCC | AGATTCGTAGCTGGATGCCG |
IL6 | ATGAACTCCTTCTCCACAAGCG | CTCCTTTCTCAGGGCTGAG |
IL8 | TTCTGCAGCTCTGTGTGAAGG | TAATTTCTGTGTTGGCGCAGTG |