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Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Involvement of lncRNA IL21-AS1 in interleukin-2 and T follicular regulatory cell activation in systemic lupus erythematosus

Fig. 3

IL21-AS1 is positively associated with IL-2 expression and activated Tfr cells. A The relative mRNA expression levels of IL21-AS1, IL2, and IL21 were analyzed in the isolated PBMCs of patients with SLE (n = 53). Correlation of IL21-AS1 level with IL2 and IL21 in patients with SLE. B, C PBMCs were isolated from the peripheral blood of patients with SLE (n = 29), and they were analyzed using flow cytometry without incubation and qPCR. B Gating strategy was employed to identify Tfh (CD4+CXCR5+Foxp3), Tfr (CD4+CXCR5+Foxp3+), Tconv (CD4+CXCR5Foxp3), PD-1hi Tfh (CD4+CXCR5+Foxp3-PD-1hi), Tph (CD4+CXCR5-Foxp3PD-1hi), rTfr (CD4+CXCR5+CD45RA+Foxp3lo), aTfr (CD4+CXCR5+CD45RAFoxp3hi), and ns-Tfr (CD4+CXCR5+CD45RAFoxp3lo) cells. C Correlation of IL21-AS1 level with the percentage of PD-1hi Tfh cell, Tph cell, Tfr cell subsets, and aTfr/PD-1hi Tfh ratio in patients with SLE (n = 29). Data symbols represent individual subjects; Spearman’s test was used for the correlation analysis between two variables of interest

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