Fig. 2

Ad-shIRAK4 alleviated the degree of synovitis in the osteoarthritis rabbit model. A, B H&E staining was applied to assess histopathological changes in the synovial membrane. Blue arrowheads indicate the enlarged lining cell layer, green arrowheads indicate angiogenesis and yellow arrowheads indicate inflammatory cell infiltration. The pathological score of synovitis was used to quantitatively assess the severity of synovitis. C The levels of IL-1β and TNF-α in the synovial fluid were measured by ELISA. Data are expressed as the mean ± SD. Statistical analysis was performed by one-way ANOVA, ^#P < 0.05 vs. control group; *P <0.05, **P <0.01, ***P <0.0001 vs. OA model group. n=5 per group