Fig. 3

Human AF matrix biochemical characterization with herniation progression. A Histological/IHC staining for a–d: Alcian Blue/Picro-Sirius Red, scale bar: 100 μm; e–h: Collagen I, scale bar: 100 μm; i–l: Collagen II, scale bar: 100 μm; m–p: Fibronectin, scale bar: 100 μm. B Quantification of each staining per herniation type. Data presented using dot plots, with median and interquartile range. Kruskal-Wallis test followed by corrected Dunn’s were performed. *p < 0.05; **p < 0.01. C Multivariate analysis of interaction of hernia containment level with age for each staining