Fig. 2

HCQ exacerbates renal injury in the mice with proteinuric LN subjected to AKI. A Representative PAS staining images and immunohistochemical staining of renal KIM-1 in the mice with proteinuric LN subjected to cisplatin-induced AKI. B Survival rate of the mice with proteinuric LN subjected to cisplatin-induced AKI. CIS + saline (n = 16) and CIS + HCQ (n = 18). C Scr levels in the mice with proteinuric LN subjected to cisplatin-induced AKI. D Quantitative analysis of renal injury scores of the mice with proteinuric LN subjected to cisplatin-induced AKI. E Western blotting of renal KIM-1 in the mice with proteinuric LN subjected to cisplatin-induced AKI. F Quantitative analysis of KIM-1 expression in the mice with proteinuric LN subjected to cisplatin-induced AKI by Western blotting. G Representative PAS staining images and immunohistochemical staining of renal KIM-1 in the mice with proteinuric LN subjected to I/R-induced AKI. H Quantitative analysis of the renal injury scores of the mice with proteinuric LN subjected to I/R-induced AKI. I Quantitative analysis of renal KIM-1 in the mice with proteinuric LN subjected to I/R-induced AKI. J Western blotting analysis of renal KIM-1 in the mice with proteinuric LN subjected to I/R-induced AKI. K Quantitative analysis of KIM-1 expression in the mice with proteinuric LN subjected to I/R-induced AKI by Western blotting. Each bar represents the mean ± SEM. For the cisplatin-induced AKI experiments, there were more than 7 mice in each group; for the I/R-induced AKI experiments, there were more than 5 mice in each group at each time point. *P < 0.05, **P < 0.01, and ***P < 0.001. Original magnification × 200