Fig. 6

HCQ impairs the autophagy-lysosomal pathway and accelerates senescence in PTECs. A Representative immunohistochemical staining of p62 and SA-β-gal in the mice with proteinuric LN. B Quantitative analysis of the SA-β-gal-positive tubular area. C, D Western blotting analysis and quantitative analysis of renal p62 in the mice with proteinuric LN. E–H Western blotting analysis and quantitative analysis of renal p62 in the mice with proteinuric LN subjected to unilateral I/R-induced AKI. I–K Western blotting analysis and quantitative analysis of LC3 and p62 in HK-2 cells. L Flow cytometric analysis of DQ-ovalbumin staining in HK-2 cells after exposure to HCQ. J, K Flow cytometric analysis of DQ-ovalbumin staining in HK-2 cells after exposure to urinary proteins from patients with LN and HCQ. L Detection of cathepsin B activity in HK-2 cells after exposure to urinary proteins from patients with LN and HCQ. Each bar represents the mean ± SEM of at least 3 independent experiments, with more than 5 mice in each group at each time point. *P < 0.05, **P < 0.01, and ***P < 0.001