Fig. 6

Acid sphigomyelinase is a target of penfluridol. A Raw 264.7 cells were collected to perform DARTS assay. B Raw 264.7 cells were used to conducted ASM activity assay. Cells were treated in presence or absence of TNFα (10 ng/mL) overnight, then lysed by freeze-thaw in liquid nitrogen for three cycles, followed by collecting supernatants after centrifuging for activity assay. C–J Flow cytometry to test effects of penfluridol on spleen naïve CD4+T cell differentiation into T cell subsets. C Differentiation from naïve CD4+T to TH1. D Quantification of C. E Differentiation from naïve CD4+T to TH2. F Quantification of E. G Differentiation from naïve CD4+T to TH17. H Quantification of G. I Differentiation from naïve CD4+T to Treg. J quantification of I. Experiments were performed for 3 biological replications and one-way analysis of variance and Bonferroni post hoc test were used to test statistical significance of the differences among groups. (NS = not significance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). PF: penfluridol