Table 3 Differentially regulated genes in TNF-Tg vs WT mice specific to LMCs. Lbp, Mcoln2, Slc16a2, Adamts1, and Calr are the top 5 upregulated genes that are specific to tumor necrosis factor transgenic (TNF-Tg) lymphatic muscle cells (LMCs) without notable differential gene expression in TNF-Tg vascular smooth muscle cells (VSMCs) (bold). Similarly, Edn1, Thy1, Fbn1, Cyp2f2, and Clic5 are the top 5 genes specifically downregulated in TNF-Tg LMCs (not bold). Proteins coded by the gene, log2 fold-change (log2FC), and false discovery rates (FDRs) are provided. The top 5 genes were ranked based on log2FC effect size with an FDR < 0.01. The genes were selected by evaluating the top up- or downregulated genes for LMCs and listed if the particular genes were unique to LMCs and not also differentially regulated in VSMCs. The LMC and VSMC populations were defined based on the wild-type (WT) and TNF-Tg integrated datasets in Fig. 3
Gene | Protein | Avg Log2FC | FDR |
|---|---|---|---|
Lbp | Lipopolysaccharide-Binding Protein | 0.79 | 3.05E−31 |
Mcoln2 | Mucolipin-2 | 0.47 | 1.03E−46 |
Slc16a2 | Monocarboxylate Transporter 8 | 0.46 | 1.14E−30 |
Adamts1 | A Disintegrin and Metalloproteinase with Thrombospondin Motifs 1 | 0.46 | 3.81E−08 |
Calr | Calreticulin | 0.45 | 6.97E−22 |
Edn1 | Endothelin-1 | − 1.14 | 2.45E−42 |
Thy1 | Thy-1/CD90 | − 0.69 | 4.05E−29 |
Fbn1 | Fibrillin-1 | − 0.64 | 2.14E−33 |
Cyp2f2 | Cytochrome P450/Family 2/Subfamily F/Polypeptide 2 | − 0.57 | 1.48E−12 |
Clic5 | Chloride Intracellular Channel Protein 5 | − 0.54 | 4.16E−38 |