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Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: Myeloid cell-specific deletion of Capns1 prevents macrophage polarization toward the M1 phenotype and reduces interstitial lung disease in the bleomycin model of systemic sclerosis

Fig. 6

Flow cytometry analysis of M1 and M2 macrophages in lung tissues of the bleomycin model of SSc mice. A Single cell suspensions of lung tissues from sham treated (Control) or BLM treated (bleomycin model of SSc) WT or Capns1-ko mice were subjected to flow cytometry. F4/80+ macrophages were assessed for expression of MHCII and CD206 as markers of M1 and M2 macrophages, respectively. The number of M1 macrophages was significantly higher in WT mice of the bleomycin model of SSc model and decreased in Capns1-ko mice in the bleomycin model of SSc. The number of M2 macrophages was not significantly higher in WT mice of the bleomycin model of SSc and increased significantly in Capns1-ko mice in the bleomycin model of SSc(A). Also, it was further demonstrated by immunohistochemical analyses for CD206 and MHC-II B. Scale bars, 50 μm.The MHC-II+cells were decreased in bleomycin model of SSc compared with the sham treated mice C, but the CD206+cells was similar between bleomycin model of SSc and sham treated mice D. The test of Homogeneity of variance showed P < 0.1, nonparametric test was applied. Data are mean ± SD. n = 8, *: P < 0.05, vs. sham treated WT mice; #: P < 0.05, vs. bleomycin model of SSc WT mice. OD optical density

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Arthritis Research & Therapy

ISSN: 1478-6362

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