Fig. 2

HIIT improves fatigue resistance in skeletal muscle of EAM mice. A–C Representative torque records during the in vivo fatigue protocol (70 Hz, 350 ms tetani every 3 s) of the plantar flexor muscles from control (CNT) and EAM mice with or without high-intensity interval training (HIIT). D Mean (± SEM) relative tetanic torque during fatiguing stimulation. Torque in the first tetanus was set to 100% in each muscle. Two-way repeated-measures ANOVA with Bonferroni post hoc test was performed. *P < 0.05 CNT vs EAM, ^#P < 0.05 EAM vs EAM + HIIT. E Blots showing electrophoretically separated myosin heavy chain (MyHC) isoforms in the gastrocnemius muscles in each group. F Distribution of MyHC isoforms. Data show the mean and SEM results from 6 muscles per group. One-way ANOVA was performed