Fig. 3

Lymphocytes are enriched and maintain cytokine production functionality after APS processing. a Gating schematic of T cell (CD3⁺) and B cell (CD19⁺) surface staining panel. Quantification of T and B cell concentration in APS and WBCs on a log scale. b Flow cytometry plot of CD8⁺ and CD4⁺ T cells and quantification of CD4⁺ and CD8⁺ T cell concentrations in APC and WBCs on a log scale. c Flow cytometry plots of intracellular cytokine staining results for IFN𝛄⁺, IL-17A⁺, and IL-4⁺ CD4⁺ T cells in APS and WBCs and quantification of IFN𝛄⁺, IL-17𝛂⁺, and IL-4⁺ CD4⁺ T cell concentration in APS and WBCs. d Flow cytometry plots of intracellular cytokine staining results for IFN𝛄⁺CD8⁺ T cells in APS and WBCs and quantification IFN𝛄⁺CD8⁺ T cell concentration in APS and WBCs. All data are mean with ± SD. Multiple unpaired t-test using Holm-Sidak method with set p-value threshold, alpha = 0.05 for a–c. Unpaired t-test with two-tailed p-value, alpha = 0.05 (d). *p < 0.05, **p < 0.001