Fig. 3

Downregulation of CD64/FcγRI via abatacept binding to CD86 on monocytes. The effects of anti-CD80, anti-CD86, both mAbs, or isotype-matched mAbs on abatacept-induced downregulation of CD64/FcγRI were examined in a short-term monocyte culture. Peripheral blood samples were obtained from 5 patients with RA and were used. The cell surface expression of CD64/FcγR is expressed as the mean fluorescence intensity (MFI) ratio. The results are shown in boxplots, and statistical comparisons between the two groups were made using the Mann–Whitney U test. A representative result of 3 independent experiments is shown