Fig. 2

CircPRKCH and HGF were increased, but miR-145 was decreased in IL-1β-treated chondrocytes. Primary human chondrocytes were isolated and treated with 10 ng/ml IL-1β to induce an OA cell model. A MTT assay was used to examine chondrocyte viability in different time points. B, C Flow cytometry was used to analyze chondrocytes apoptosis. D, E Western blotting was used to detect MMP-3, MMP-13, Aggrecan, and Collagen II expression. F RT-qPCR was used to detect the expression levels of circPRKCH, miR-145, and HGF levels. G, H Western blotting was used to test the protein levels of HGF, p-c-MET, and c-MET. Data are the means ± SD for three independent experiments. *p< 0.05, **p< 0.01, and ***p< 0.001