Fig. 5

MiR-145 overexpression inhibited ECM degradation by inhibiting the HGF/c-MET signaling pathway. Chondrocytes were transfected with miR-145 mimics or HGF overexpressing vector and treated with IL-1β. A, B Flow cytometry was used to determine chondrocyte apoptosis. C, D Western blot assay was used to measure MMP-3, MMP-13, Aggrecan, and Collagen II levels. Data are the means ± SD for three independent experiments. **p< 0.01 and ***p< 0.001