Fig. 1

Establishment of the NP cell degeneration model. Collagen II and aggrecan protein expression levels in primary cells were detected by immunofluorescence. B Cells were stained with HE. C MTT was used to detect cell activity. D The expression levels of cleaved caspase-3 (cl-cas-3), total caspase-3 (t-cas-3), Bax, Bcl-2, and apoptosis-related proteins were detected by Western blotting. E The protein expression levels of collagen II, aggrecan, MMP-13, and MMP-3 were detected by Western blotting. F, G show the statistical quantification results of the optical density values of the bands in the Western blotting results in D. H, I show the statistical quantification results of the optical density of the bands in the Western blot results in E. *P<0.05, ** P<0.01, compared with the control group