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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Factors secreted by monosodium urate crystal-stimulated macrophages promote a proinflammatory state in osteoblasts: a potential indirect mechanism of bone erosion in gout

Fig. 1

Conditioned medium from MSU crystal-stimulated RAW264.7 macrophages—proinflammatory factors and effect on MC3T3-E1 cell viability. RAW264.7 macrophages were cultured with MSU crystals for 24 h. A The concentrations of proinflammatory mediators secreted from MSU crystal-stimulated RAW264.7 cells, measured by ELISA. B Conditioned media preparations were added to MC3T3-E1 cells at 40% of the final culture volume. MC3T3-E1 cell viability was determined by the alamarBlue® assay. Means (SEM) of data pooled from three biological repeats are presented. Data were analyzed by one-way ANOVA with post hoc Dunnett’s test. **p < 0.01 and ***p < 0.001 versus control conditioned medium. MSU CM, concentrations of MSU crystals used to prepare conditioned medium from RAW264.7 cells

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Arthritis Research & Therapy

ISSN: 1478-6362

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