Fig. 5

Cytokine receptor gene expression in culture with synoviocytes. PBMCs and synoviocytes were cultured alone or in co-culture at a 5:1 ratio for 24 h, in the presence or absence of PHA (5 μg/ml) or IL-23 (50 ng/ml). RNA was recovered, and RT-qPCR was performed to analyze IL-17RA, IL-17RC, IL-12Rβ1, IL-12Rβ2, and IL-23R expression. The results showed the gene expression normalized to GAPDH expression. “*” compares the condition of culture (PHA or IL-23 vs. control). *p < 0.05, Wilcoxon test. n = 6 experiments