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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Augmented PFKFB3-mediated glycolysis by interferon-γ promotes inflammatory M1 polarization through the JAK2/STAT1 pathway in local vascular inflammation in Takayasu arteritis

Fig. 2

Relationship between interferon (IFN)-γ and 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) in M1 macrophages in vitro. A, B CD14.+ cell selection by flow cytometry (n = 3; con, control; ps, CD14-positive selected cells); C, D expression of PFKFB3 in M1 (induced by 20 ng/ml IFN-γ and 50 ng/ml LPS) and M2 (induced by 20 ng/ml IL-4 and 20 ng/ml IL-13) macrophage subtypes by quantitative reverse transcription PCR and flow cytometry; E effect of different concentrations of IFN-γ (0, 2, 20, 200 ng/ml) with LPS (50 ng/ml) on the expression of PFKFB3 in M1 differentiation. Data revealed as mean ± SEM, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001

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Arthritis Research & Therapy

ISSN: 1478-6362

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