Fig. 2

circ-Iqsec1 plays a role during osteogenic BMSC differentiation. A ALP staining shows the BMSC osteogenic differentiation post osteogenic induction for 0, 2, and 3 weeks. B Alizarin red staining showing BMSC osteogenic differentiation post-osteogenic induction for 0, 2, and 3 weeks. C NGS was used for circRNA expression detection in BMSCs after osteogenic induction for 0 (NC) and 3 (T) weeks. D RT-qPCR detection showing abnormal circRNA expression. Results are denoted by the mean ± SD. ^**P < 0.01, ^***P < 0.001 vs. NC. E RT-qPCR data giving circ-Iqsec1 expression during osteogenic differentiation of BMSCs. Output is denoted as the mean ± SD. ^**P < 0.01, ^***P < 0.001 vs. 0 day. F Genomic loci of Iqsec1 and mmu_circ_0001493