Fig. 5

The miR-187-3p links Satb2 and circ-Iqsec1. A Venn diagram showing that miRNA interacts with both Satb2 and circ-Iqsec1. B RT-qPCR data giving nine miRNA expressions in BMSCs post osteogenic induction for 0 (NC) and 3 (T) weeks. C Bioinformatics analyses predicting miR-187-3p binding sites in circ-Iqsec1. MUT version regarding circ-Iqsec1 is provided. D Relative luciferase activity 2 d post HEK293T cell transfection with miR-187-3p mimic/NC or circ-Iqsec1 WT/MUT. E miR-187-3p binding site predictions regarding Satb2 3'-UTR. Mutant 3′-UTR-Satb2 version is given. F Relative luciferase activity 2 days post-HEK293T cell transfection through miR-187-3p mimic/NC or 3′-UTR-Satb2 WT/Mut. G–I RT-qPCR results giving circ-Iqsec1, miR-187-3p, and Satb2 expressions in BMSCs after transfection with si-circ-Iqsec1, miR-187-3p inhibitor, or Satb2 overexpression vector alone or in combination. Outputs are expressed as the mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. NC. ^###P < 0.001 vs. si-circ-Iqsec1