Fig. 3

Ameliorated nephritis in mice with Axl-targeted early intervention. Three groups of mice (B6 (n = 6), B6.Axl-KO (n = 6), and B6 + R428 (n = 7)) were included. Nephritis was induced in experimental mice. The experiment was terminated on day 21. Blood, urine, and kidney samples were collected and processed for further analysis. A Schematic depiction of the treatment option with start time, dose, and interval. B Representative images for anti-sheep IgG immunofluorescent staining. C Quantitative analysis of the immunofluorescent intensity. D BUN levels were analyzed as depicted in the “Materials and methods” section. Kidney sections were processed for histology analysis (E, F). G CD4⁺ T cells were stained, and images were taken. T cell numbers per microscopic field were counted, and averaged numbers from each mouse were presented in H. Experiments were repeated three times. Similar data were obtained, and one set was presented here. Statistical analysis was performed with an unpaired t test with Welch’s correction. ns, not significant; **p < 0.01; ***p < 0.001. Representative images were shown at × 200 magnification