Fig. 3

Naringenin decreased the expression of IBA-1 in the spinal cord dorsal horn in CIA rats. A The expression of IBA-1 in the spinal cord dorsal horn on days 28 (PID 21) and 42 (PID 35) were examined by immunofluorescence. B, C Expression of IBA-1 in the spinal cord dorsal horn in CIA and NAR groups on the day 28 (PID 21) and day 42 (PID 35). D, E Representative Western blots showing the expression of IBA-1 in the spinal cord dorsal horn in CIA and NAR groups on days 28 (PID 21) and 42 (PID 35). *P < 0.05, **P < 0.01 vs. naive group, ^#P < 0.05, ^##P < 0.01 vs. CIA model group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. All scale bars are 50 μm. The relative mean fluorescence intensity of IBA-1 was quantified using the Image J software. The data are presented as means ± SEM (n = 5 rats per group)