Fig. 6

Naringenin reduces phosphorylation of CRMP2 in spinal cord dorsal horn of CIA rats on day 42 (PID 35) following the first day of initial immunization. A, B The expression levels of CRMP2 and pCRMP2 S522 in the spinal cord dorsal horn on day 42 (PID 35) were examined by immunofluorescence. Representative Western blots showing expression of CRMP2 (C), CDK5 (D), pCRMP2 S522 (E), and pCRMP2 Y479 (F) in the spinal cord dorsal horn in CIA and NAR groups on day 42 (PID 35). G Representative blots are shown. *P < 0.05 vs. naive group, **P < 0.01 vs. naive group, ^#P < 0.05 vs. CIA model group, ^##P < 0.01 vs. CIA model group, by repeated-measures one-way ANOVA followed by post hoc Dunnett’s multiple comparisons test. The data are presented as means ± SEM (n = 5 rats per group). All scale bars are 50 μm. The relative mean fluorescence intensity of pCRMP2 S522 were quantified using the Image J software. The data are presented as means ± SEM (n = 5 rats per group)