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Fig. 1 | Arthritis Research & Therapy

Fig. 1

From: Implementation of automated behavior metrics to evaluate voluntary wheel running effects on inflammatory-erosive arthritis and interstitial lung disease in TNF-Tg mice

Fig. 1

Automated wheel running metrics reveal a progressive decline in running performance for TNF-Tg mice. Wheel-enriched cages (pink arrow) were placed adjacent to a workstation (white arrow) with USB compatibility (A). A reed switch (blue arrow) wired to the workstation interface passed through a small gap in the cage lid, and securely attached to the base of the wheel. The wheel (green arrow) had a neodymium magnet attached to the outside rim facing the reed switch, such that each rotation of the wheel closed the reed switch (B). At 2 months of age, WT and TNF-Tg mice were placed individually in this enriched housing environment and provided free access to the running wheel. Daily activity metrics were collected and then transformed into 10-day medians synchronized to the date of birth. Median distance plots are provided for all groups (C–F) and showed running distance is dependent on genotype, sex, and time. In addition, derived metrics such as median running rate (G), running bout length (H), and number of runs (I) were also dependent on genotype and time, while sex was non-contributory. Statistics: 3-way mixed-effects; *p<0.05, ****p<0.0001

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Arthritis Research & Therapy

ISSN: 1478-6362

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