Equine osteoarthritis modifies fatty acid signatures in synovial fluid and its extracellular vesicles

Table 1 Original p values from the generalized linear model (GLM) of the synovial fluid (SF) and its extracellular vesicle-enriched pellet (EV) fatty acids (FAs) and the calculated Benjamini–Hochberg (B–H) critical values used to control the false discovery rate

SF FA	p group	SF FA	p group × age	SF B–H critical value	EV FA	p group	EV FA	p group × age	EV B–H critical value
n-3/n-6 PUFAs	3 × 10^–14*	n-3/n-6 PUFAs	3 × 10^–12*	0.0005	18:0	0.002	22:0	0.002	0.0006
16:1n-7	7 × 10^–11*	16:1n-7	1 × 10^–8*	0.0011	22:0	0.003	18:0	0.004	0.0011
17:0i	3 × 10^–8*	17:0i	1.5 × 10^–7*	0.0016	16:0	0.020	16:1n-7	0.032	0.0017
Prod/prec n-6	0.00008*	Prod/prec n-6	0.0005*	0.0022	∆5-DI n-6	0.040	16:0	0.077	0.0022
18:2n-6	0.0006*	16:0i	0.0024*	0.0027	22:1n-9	0.040	22:1n-9	0.079	0.0028
18:0i	0.001*	18:0i	0.0031*	0.0033	20:4n-6	0.088	18:2n-6	0.097	0.0033
16:0i	0.001*	18:2n-6	0.0037*	0.0038	16:1n-9	0.091	18:3n-3	0.109	0.0039
∆5-DI n-6	0.003*	24:1n-9	0.0039*	0.0043	16:1n-7	0.093	Prod/prec n-3	0.139	0.0044
14:0	0.003*	22:0	0.0047*	0.0049	17:0i	0.108	15:0	0.162	0.0050
n-6 PUFAs	0.004*	∆5-DI n-6	0.010	0.0054	15:0	0.109	16:1n-9	0.187	0.0056
22:0	0.004*	n-6 PUFAs	0.012	0.0060	18:2n-6	0.121	∆5-DI n-6	0.202	0.0061
DMA 18:0	0.005*	DMA 18:0	0.015	0.0065	20:0	0.130	20:0	0.205	0.0067
24:1n-9	0.013	20:3n-6	0.020	0.0071	DMA 18:0	0.132	DBI	0.222	0.0072
∆6-DI n-6	0.019	n-3 PUFAs	0.029	0.0076	18:3n-3	0.133	SFAs	0.284	0.0078
n-3 PUFAs	0.023	14:0	0.029	0.0082	DMAs	0.172	UFAs/SFAs	0.312	0.0083
18:3n-6	0.032	18:3n-6	0.046	0.0087	24:0	0.198	DMAs	0.355	0.0089
20:3n-6	0.033	Prod/prec n-3	0.054	0.0092	Prod/prec n-3	0.199	17:0ai	0.412	0.0094
17:0ai	0.043	22:5n-3	0.054	0.0098	DBI	0.208	20:4n-6	0.437	0.0100

The p values that remained significant after the procedure were obtained by comparing the original p value to the corresponding critical value on the same row. Asterisk indicates comparisons for which the direction of the difference is confidently interpreted at the α/2 level. The SF FAs that originally had p values >0.05 in the GLM were excluded from the table. In the EV fraction, no significant differences remained after the procedure, but a similar number of FAs is shown
ai anteiso-methyl-branch, DBI double bond index, DI desaturation index, DMA dimethyl acetal, i iso-methyl-branch, Prod/prec product/precursor ratio, PUFA polyunsaturated fatty acid, SFA saturated fatty acid, UFA unsaturated fatty acid

ISSN: 1478-6362