Fig. 4

Protein expression of pSMAD2 (Ser465/467) in urate primed monocytes. The monocytes were primed overnight with no, 6.25 or 12.5 mg/dL urate followed with a stimulation of 1 ng/mL TGFβ for 1 h. Cell lysates were used for western blotting. Relative pSMAD2 expression (n = 7) (A) and a representative blot (B) are shown. Wilcoxon signed-rank test was used to compare means. *p < 0.05