Fig. 2

MYC promoted fibroblast transdifferentiation by upregulating ALP and BMP2. a Fibroblasts were infected with negative control lentivirus (NC) and MYC knockdown lentivirus (MYC-KD) and cultured in ODM for 10 or 20 days. The expression levels of the transcription factor MYC and osteogenic genes were measured by q-PCR (n = 4). b ALP was stained in fibroblasts cultured in ODM from NC and MYC-KD groups on D20, shown in the culture dish (upper panel) and under the microscope (lower panel). Scale bar = 200 µm. c Levels of mineralization in fibroblasts were measured in NC and MYC-KD groups on D20 by OsteoImageTM Mineralization Assay, shown in light field (upper panel) and fluorescent light (lower panel) microscopically. Scale bar = 100 µm. d The interactions between the transcription factor MYC and osteogenic genes were confirmed by ChIP, and representative gels of the amplified promoters of ALP and BMP2 are shown in the left panel. The grouping of gels/blots was cropped from different gels. No high contrast (overexposure) of blots was performed. Fold enrichment of ALP and BMP2 was calculated over negative control mouse IgG (right panel). Values are reported as the mean ± SEM. *p < 0.05. **p < 0.01