Fig. 6

FTO inhibited LPS-induced chondrocyte damage through upregulating PRKAA2. Chondrocytes were transfected with FTO overexpression or shPRKAA2 or a combination of FTO overexpression and shPRKAA2 and then treated with 40 ng/mL LPS for 24 h. A CCK-8 was used to detect the viability of chondrocytes. B EDU staining was applied to detect the proliferation ability of chondrocytes. D and F The apoptosis of chondrocytes was assessed by flow cytometry. E The caspase-3 activity was measured by caspase-3 activity kit. F Western Blot was applied to assess the protein expression of MMP13, ADAMTS5, Aggrecan, and COL2A1. LPS, lipopolysaccharide; shPRKAA2, PRKAA2 knockdown adenovirus; FTO, FTO overexpression adenovirus; n = 3, *p < 0.05, **p < 0.01, ***p < 0.001