Fig. 7
From: FTO-mediated m6A demethylation of pri-miR-3591 alleviates osteoarthritis progression
FTO ameliorated the OA progression in mice. A The protein expression of FTO, MMP13, ADAMTS5, Aggrecan, and COL2A1 was evaluated by western blotting in the knee cartilage of OA mice with or without AAV-FTO treatment. B RT-qPCR was applied to assess the expression of miR-3591-5p in the knee cartilage of OA mice with or without AAV-FTO treatment. C The section of the knee joints was stained by HE staining and Safranin O & Fast Green staining, respectively. D OARSI scores of the knee joints in OA mice with or without AAV-FTO treatment. Sham, mice without DMM; model, DMM surgery-induced OA mice; AAV-NC, negative control corresponding to FTO overexpression adeno-associated virus; AAV-FTO, FTO overexpression adeno-associated virus; n = 6, *p < 0.05, **p < 0.01, ***p < 0.001