Fig. 4

Analysis of S100A8 and S100A8/A9 expression and localization in peripheral blood B cells using confocal microscopy. Peripheral B cells were fixed and stained by antibodies against CD19 (red), nucleus (blue), and S100A8 or A8/A9 (green) by confocal microscopy. In the anti-S100A8 (3H2617) staining, the rims of B cells from patients with active SLE were stained, while B cells from the HC were not stained. The cytoplasm of monocytes was brightly stained by 3H2617, acting as the positive controls. Anti-S100A8/A9 (27E10) did not stain the B cells of patients with SLE or HC