Fig. 2

D2R agonist sumanirole ameliorates depolarization of Th1 and Th17 cells and symptoms of arthritis in CIA mice. A Flow cytometric assay of the CD4⁺ T cell subsets. The left panel represents the flow cytometric images and the right panel indicates respective percentage of the CD4⁺ T cell subsets (Th1, Th2, Th17 and Treg cells) in total CD4⁺ T cells (n = 5/group). B Protein expression of the specific transcription factors in ankle joints of mice. Representative Western blotting bands are exhibited in the left panel and quantitative values obtained by densitometric analysis were normalized to β-actin in the right panel (n = 5/group). C-F Contents of the cytokines IFN-γ, IL-4, IL-17A and TGF-β1 in ankle joints of mice measured by ELISA. All n = 5. G Clinical arthritis score of four limbs. The arrow indicates the first sumanirole administration. n = 6/group. H Hind paw thickness and ankle joint width of mice. n = 6/group. I Anti-CII IgG antibody levels in serum of mice measured by ELISA. n = 5/group. *P < 0.05, **P < 0.01, versus saline group; ^#P < 0.05, ^##P < 0.01, versus CIA + saline group; NS, no significance