Fig. 2

Validation of the altered glycopatterns in OA cartilages. a Binding patterns of glycoproteins in cartilage proteins from OA patients (n = 3) and controls (n = 3) for WGA, SJA, and ConA staining. The protein bands showed differences between OA and control cartilages marked with red frames. The expression level of GAPDH served as the loading control. b The gray value of the different protein bands was measured by ImageJ software, and the gray values of the selected bands were represented as mean with SEM. c The results of lectin histochemistry. The articular cartilage sections from healthy and OA patients were stained with Cy5-labeled PSA. The images were acquired at the same conditions. Quantification was shown to the right. Data were obtained from OA (n = 3) and control (n = 3) cartilage sections. Mean fluorescence intensities were represented as mean with SEM. Scale bar = 100 μm