Fig. 5

Expression analysis of glycosyltransferases involved in the synthesis of N-glycans. a, b The differential expression transcripts in OA-LT compared to N-LT (a) and OA-MT compared to N-MT (b) in GSE51588. c The expression levels of MGAT5B, MGAT4C, ALG3, and ALG5 showed evident differences between OA and controls. d KEGG pathway map of glycosyltransferases. ALG3 and ALG5 are involved in the synthesis of N-glycan precursor (upper), and MGAT5B and MGAT4C are involved in the synthesis of GlcNAc branching of N-glycans (lower). The linkage catalyzed by ALG3 was marked with a green line, and the linkages catalyzed by MGAT5B and MGAT4C were marked with a red line. e The results of SA-β-Gal staining. The C28 cells were treated with or without 10 ng /mL of IL-1β for 48 h and the senescent cells were stained with SA-β-Gal. Quantitative analysis of the percentage of SA-β-Gal positive cells in untreated- versus IL-1β-treated chondrocytes. Data were represented as Mean ± SEM. from three independent experiments. Scale bar = 50 μm. f The expression level of MMP-9, MMP-13, ADAMTS-4, and COL2A1 was significantly altered in C28 cells treated with IL-1β. g The expression of MGAT4C and MGAT5B was increased and the expression of ALG3 and ALG5 was decreased in C28 cells stimulated by IL-1β