Fig. 1

TUG1 is lowly expressed in human osteoarthritic primary chondrocytes. A Differential analysis of the GEO database dataset (GSE175960) using the limma package. B Identification of primary chondrocytes by Saffron-O and Fast Green, toluidine blue, and collagen II immunocytochemistry staining. C Western blotting to detect the protein expression of Bax, Bcl2, and cleaved-caspase3 under different concentrations of IL-1β stimulation. D Western blotting to detect the protein expression of Bax, Bcl2, and cleaved-caspase3 after different times of IL-1β stimulation. E Western blotting to detect the protein expression of collagen II, MMP13, Bax, Bcl2, and cleaved-caspase3 after 24-h stimulation with 10 ng/ml IL-1β. F qRT-PCR assay of TUG1 mRNA expression after 24-h stimulation of chondrocytes using 10 ng/ml IL-1β. All data are shown as SEM ± mean based on 3 independent experiments. *P < 0.05; **P < 0.01; P < 0.001