Fig. 2

TUG1 is downregulated in IL-1β-induced C28/I2 chondrocyte cell line. A Western blotting to detect the protein expression of Bax, Bcl2, and cleaved-caspase3 at different concentrations of IL-1β stimulation. B Western blotting to detect the protein expression of Bax, Bcl2, and cleaved-caspase3 after different times of IL-1β stimulation. C Western blotting to detect the protein expression of Collagen II, MMP13, Bax, Bcl2, and cleaved-caspase3 after 10 ng/ml IL-1β stimulation for 24 h. D Immunofluorescence detection of collagen II and MMP13 protein expression after 10 ng/ml IL-1β stimulation for 24 h. E qRT-PCR assay of TUG1 mRNA expression after 10 ng/ml IL-1β stimulation for 24 h. All data are shown as SEM ± mean based on 3 independent experiments. *P < 0.05; **P < 0.01; P < 0.001