Fig. 4

miR144-3P is a target miRNA for TUG1. A RNAhybrid database analysis reveals a potential interaction between TUG1 and miR144-3p. B qRT-PCR assay of miR144-3p expression in IL-1β-induced human primary chondrocytes. C qRT-PCR to detect IL-1β-induced miR144-3p expression in C28/I2 cells. D, E Targeted binding of TUG1 to miR144-3p was detected by double luciferase reporter gene after mimic and inhibitor cotransfection of TUG1-WT and TUG1-MUT with miR144-3p. F RIP assay for the binding of TUG1, AGO2 protein, and miR144-3p. G qRT-PCR assay for miR144-3p expression of Si-TUG1. All data are shown as SEM ± mean based on 3 independent experiments. *P < 0.05; **P < 0.01; P < 0.001