Fig. 5

miR144-3P promotes apoptosis and extracellular matrix degradation in chondrocytes. A Western blotting to detect the protein expression of collagen II, MMP13, Bax, Bcl2, and cleaved-caspase3 after transfection with mimic. B Western blotting to detect the protein expression of collagen II, MMP13, Bax, Bcl2, and cleaved-caspase3 after transfection with inhibitor. C Immunofluorescence detection of collagen II and MMP13 protein expression after transfection with mimic, inhibitor. D Hochest33342 assay for apoptosis after transfection with mimic, inhibitor. All data are shown as SEM ± mean based on 3 independent experiments. *P < 0.05; **P < 0.01; P < 0.001