Fig. 6

DUSP1 is the target mRNA of miR144-3P. A Western blotting to detect the protein expression of DUSP1 in IL-1β-induced human primary chondrocytes. B qRT-PCR to detect IL-1β-induced mRNA expression of DUSP1 in human primary chondrocytes. C Western blotting to detect the protein expression of DUSP1 in IL-1β-induced C28/I2 cells. D qRT-PCR to detect the mRNA expression of DUSP1 in IL-1β-induced C28/I2 cells. E RNAhybrid database analysis revealed a potential interaction between miR144-3P and DUSP1. F RIP assay for targeted binding of miR144-3P to DUSP1. G qRT-PCR to detect the mRNA expression level of DUSP1 after transfection with mimic, inhibitor. H Western blotting detection of protein expression levels of DUSP1 after transfection with mimic, inhibitor. All data are shown as SEM ± mean based on 3 independent experiments. *P < 0.05; **P < 0.01; P < 0.001