Fig. 8

TUG1 regulates the course of osteoarthritis through the miR144-3P/DUSP1/p38 MAPK axis. A qRT-PCR to detect DUSP1 mRNA expression after transfection with Si-TUG1. B Western blotting to detect the protein expression of DUSP1 after transfection with Si-TUG1. C Western blotting to detect IL-1β-induced protein expression of P38 and P-P38 in chondrocytes. D Western blotting to detect P38 and P-P38 protein expression after transfection with AD-DUSP1. E Western blotting to detect the protein expression of collagen II, MMP13, Bax, and Bcl2 after cotransfection with Si-TUG1, miR144-3P inhibitor. F Western blotting to detect the protein expression of collagen II, MMP13, Bax, and Bcl2 after miR144-3P mimic, AD-DUSP1 cotransfection. G Western blotting to detect the protein expression of collagen II, MMP13, Bax, and Bcl2 after cotransfection with Si-TUG1, AD-DUSP1. All data are shown as SEM ± mean based on 3 independent experiments. *P < 0.05; **P < 0.01; P < 0.001