Fig. 4

Knockdown of Piezo1 reduced the apoptosis induced by CMS. a Representative flow cytometry scatter plots detecting AFCs apoptosis in the Ctrl group, CMS group, CMS + Lv-Ctrl group, and CMS + Lv-Piezo1 group. b Representative Tunel staining fluorescence pictures detecting AFCs apoptosis in the Ctrl group, CMS group, CMS + Lv-Ctrl group, and CMS + Lv-Piezo1 group (scale bar = 50 μm). Apoptotic AFCs appeared red and nuclei were counterstained with Hoechst 33,358 (blue). c Representative MMP staining fluorescence pictures detecting AFCs apoptosis in the Ctrl group, CMS group, CMS + Lv-Ctrl group, and CMS + Lv-Piezo1 group (scale bar = 50 μm). MMP appeared red and nuclei were counterstained with Hoechst 33,358 (blue). d Statistic data of apoptotic AFCs in the Ctrl group, CMS group, CMS + Lv-Ctrl group, and CMS + Lv-Piezo1 group determined by flow cytometry (n = 3). e Statistic data of Tunel-positive apoptotic AFCs in the Ctrl group, CMS group, CMS + Lv-Ctrl group, and CMS + Lv-Piezo1 group determined by Tunel staining (n = 3). f Statistic data of mean MMP fluorescent intensity in AFCs in the Ctrl group, CMS group, CMS + Lv-Ctrl group, and CMS + Lv-Piezo1 group determined by MMP staining (n = 3). ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001