Fig. 3

Drp1 activates NF-κB pathway and NLRP3 inflammasome by modulating mitochondrial fission. A TEM analysis of mitochondria from RAW264.7 and J774A.1 cells. Scale bar = 500 nm. B Mitochondrial ROS was assessed by MitoROX staining. Red, MitoROX. Scale bar = 100 μm. C The protein levels of Drp1 and key molecules of NF-κB/NLRP3 signaling were detected by western blot. *, P < 0.05, **, P < 0.01, ***, P < 0.001