Fig. 10
From: Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression
Sirt3 deficiency aggravated MSU crystal-induced inflammatory cell infiltration and inflammation-related protein expression in the paw of mice. a The distribution of Ly-6G, CCR2, and MPO-positive cells in mouse paw tissue sections was detected by immunofluorescence assay. Blue shows nuclei stained with DAPI. Scale bar, 80 μm. n = 5 mice per group. Ten to 12 images per mouse were analyzed. b Representative Western blot and statistical analysis of protein levels of CCL2, NLRP3, ACOD1, MPO, SIRT3, and CCR2 in mouse paw tissue homogenates. Data presented as mean ± SD (n = 5 mice per group). *P < 0.05