Fig. 2

Sirt3 deficiency increased mtROS production and NF-κB activation in BMDMs treated with C16:0 + MSU. a Sirt3 protein levels of BMDMs from Sirt3^+/+ and Sirt3^−/− mice. b The statistical analysis graph of mitochondrial ROS generation. c Mitochondrial ROS levels were measured by LCM image using a Mito-SOX probe. Red fluorescence represented mitochondrial ROS level. Blue shows nuclei staining with DAPI. Scale bar: 40 μm. d Mitochondrial respiration (oxygen consumption rate, OCR) was measured using Seahorse XFe24 Analyzer. e Quantification of basal respiration, maximal respiration, and OCR-coupled ATP production were analyzed (n = 5 biological replicates). f ATP Lite Luminescence Assay kit was used to detect global ATP production (n = 4 biological replicates). g Cytosolic and nuclear protein extracted from BMDMs were respectively reacted with anti-phosphorylated NF-κB P65 (p-P65) and anti-NF-κB P65 by western blotting. *P < 0.05. NS means no statistical difference