Fig. 3
From: Sirt3 improves monosodium urate crystal-induced inflammation by suppressing Acod1 expression
In BMDMs treated with C16:0 + MSU, Sirt3 deficiency accelerated NLRP3 inflammasome activation. a The protein levels of NLRP3, Caspase-1, and IL-1β in the cell lysates were evaluated by Western blot. Mature IL-1β (p17) and p20 fragments of Caspase-1 were detected in cell culture supernatants. b Total cell lysates were obtained in Triton X-100–containing buffer. Insoluble (pellet) fractions were cross-linked with DSS to capture ASC oligomers. The soluble and insoluble fractions were analyzed by Western blot with ASC antibody. c Representative LCM images of BMDMs stained with ASC antibody. DAPI staining nuclei. Scale bar, 40 μm. *P < 0.05. NS shows no statistical difference